np68 peptide 366-374  (AnaSpec)

Journal: Immunity

Article Title: PIP5 Kinases Regulate Membrane Phosphoinositide and Actin Composition for Targeted Granule Secretion by Cytotoxic Lymphocytes

doi: 10.1016/j.immuni.2018.08.017

Figure Lengend Snippet:

Article Snippet: ASNENMDAM (NP68 peptide 366-374) , Anaspec , AS-60623.

Techniques: Blocking Assay, Control, Virus, Recombinant, Electron Microscopy, Fractionation, Software

Journal:

Article Title: Effect of a Small Molecule BCL-2 Inhibitor on Immune Function and Use with a Recombinant Vaccine

doi: 10.1002/ijc.25177

Figure Lengend Snippet: CD8 T lymphocytes from TCR-Tg F5 mice were stimulated with NP68 peptide in the absence or presence of Treg cells. Tregs were purified from spleens of untreated or GX15-070-treated C57BL/6 mice. Proliferation of CD8 cells was measured by [3H]thymidine incorporation. CD4+/CD25+/FoxP3+ phenotype of Tregs was confirmed by flow cytometry.

Article Snippet: Cells were cultured for three days in complete CTL medium (RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 units/mL penicillin, and 100 µg/mL streptomycin) enriched with 10 −4 µg/mL of F5 TCR 366 ASNENMDAM 374 ligand peptide 68 (NP68) (American Peptide Company Inc., Sunnyvale, CA).

Techniques: Purification, Flow Cytometry